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anti pakt serine 473 9271 antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti pakt serine 473 9271 antibodies
    Figure 5. Differences in the activation of MAP kinases and AKT in response to PDGF-BB stimulation between pig arterial smooth muscle cells (ApSMCs) and venous smooth muscle cells (VpSMCs). (A,E) Confluent cells were serum-starved overnight and stimulated with PDGF-BB at the indicated time points. Cell lysates were analyzed for the expression of the indicated proteins via immunoblots. (B–D,F) Individual band densitometry data were obtained using Image J (NIH version 1.53K). The ratio values were obtained by dividing the band densitometry values of pERK1/2 (B), pJNK (C), pP38 (D) and <t>pAKT</t> (F) by the loading protein values (n = 4 for pERK1/2 and pJNK; n = 3 for pP38; n = 5 for pAKT). ****: p < 0.0001; ***: p < 0.001; **: p < 0.01; *: p < 0.05; ns: non-significant differences were assessed using an ANOVA test.
    Anti Pakt Serine 473 9271 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 33184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Characterization of Protein Expression and Signaling Pathway Activation That May Contribute to Differential Biological Functions in Porcine Arterial and Venous Smooth Muscle Cells."

    Article Title: Characterization of Protein Expression and Signaling Pathway Activation That May Contribute to Differential Biological Functions in Porcine Arterial and Venous Smooth Muscle Cells.

    Journal: International journal of molecular sciences

    doi: 10.3390/ijms26073110

    Figure 5. Differences in the activation of MAP kinases and AKT in response to PDGF-BB stimulation between pig arterial smooth muscle cells (ApSMCs) and venous smooth muscle cells (VpSMCs). (A,E) Confluent cells were serum-starved overnight and stimulated with PDGF-BB at the indicated time points. Cell lysates were analyzed for the expression of the indicated proteins via immunoblots. (B–D,F) Individual band densitometry data were obtained using Image J (NIH version 1.53K). The ratio values were obtained by dividing the band densitometry values of pERK1/2 (B), pJNK (C), pP38 (D) and pAKT (F) by the loading protein values (n = 4 for pERK1/2 and pJNK; n = 3 for pP38; n = 5 for pAKT). ****: p < 0.0001; ***: p < 0.001; **: p < 0.01; *: p < 0.05; ns: non-significant differences were assessed using an ANOVA test.
    Figure Legend Snippet: Figure 5. Differences in the activation of MAP kinases and AKT in response to PDGF-BB stimulation between pig arterial smooth muscle cells (ApSMCs) and venous smooth muscle cells (VpSMCs). (A,E) Confluent cells were serum-starved overnight and stimulated with PDGF-BB at the indicated time points. Cell lysates were analyzed for the expression of the indicated proteins via immunoblots. (B–D,F) Individual band densitometry data were obtained using Image J (NIH version 1.53K). The ratio values were obtained by dividing the band densitometry values of pERK1/2 (B), pJNK (C), pP38 (D) and pAKT (F) by the loading protein values (n = 4 for pERK1/2 and pJNK; n = 3 for pP38; n = 5 for pAKT). ****: p < 0.0001; ***: p < 0.001; **: p < 0.01; *: p < 0.05; ns: non-significant differences were assessed using an ANOVA test.

    Techniques Used: Activation Assay, Expressing, Western Blot



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    Figure 5. Differences in the activation of MAP kinases and AKT in response to PDGF-BB stimulation between pig arterial smooth muscle cells (ApSMCs) and venous smooth muscle cells (VpSMCs). (A,E) Confluent cells were serum-starved overnight and stimulated with PDGF-BB at the indicated time points. Cell lysates were analyzed for the expression of the indicated proteins via immunoblots. (B–D,F) Individual band densitometry data were obtained using Image J (NIH version 1.53K). The ratio values were obtained by dividing the band densitometry values of pERK1/2 (B), pJNK (C), pP38 (D) and <t>pAKT</t> (F) by the loading protein values (n = 4 for pERK1/2 and pJNK; n = 3 for pP38; n = 5 for pAKT). ****: p < 0.0001; ***: p < 0.001; **: p < 0.01; *: p < 0.05; ns: non-significant differences were assessed using an ANOVA test.
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    Figure 5. Differences in the activation of MAP kinases and AKT in response to PDGF-BB stimulation between pig arterial smooth muscle cells (ApSMCs) and venous smooth muscle cells (VpSMCs). (A,E) Confluent cells were serum-starved overnight and stimulated with PDGF-BB at the indicated time points. Cell lysates were analyzed for the expression of the indicated proteins via immunoblots. (B–D,F) Individual band densitometry data were obtained using Image J (NIH version 1.53K). The ratio values were obtained by dividing the band densitometry values of pERK1/2 (B), pJNK (C), pP38 (D) and <t>pAKT</t> (F) by the loading protein values (n = 4 for pERK1/2 and pJNK; n = 3 for pP38; n = 5 for pAKT). ****: p < 0.0001; ***: p < 0.001; **: p < 0.01; *: p < 0.05; ns: non-significant differences were assessed using an ANOVA test.
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    Image Search Results


    Figure 5. Differences in the activation of MAP kinases and AKT in response to PDGF-BB stimulation between pig arterial smooth muscle cells (ApSMCs) and venous smooth muscle cells (VpSMCs). (A,E) Confluent cells were serum-starved overnight and stimulated with PDGF-BB at the indicated time points. Cell lysates were analyzed for the expression of the indicated proteins via immunoblots. (B–D,F) Individual band densitometry data were obtained using Image J (NIH version 1.53K). The ratio values were obtained by dividing the band densitometry values of pERK1/2 (B), pJNK (C), pP38 (D) and pAKT (F) by the loading protein values (n = 4 for pERK1/2 and pJNK; n = 3 for pP38; n = 5 for pAKT). ****: p < 0.0001; ***: p < 0.001; **: p < 0.01; *: p < 0.05; ns: non-significant differences were assessed using an ANOVA test.

    Journal: International journal of molecular sciences

    Article Title: Characterization of Protein Expression and Signaling Pathway Activation That May Contribute to Differential Biological Functions in Porcine Arterial and Venous Smooth Muscle Cells.

    doi: 10.3390/ijms26073110

    Figure Lengend Snippet: Figure 5. Differences in the activation of MAP kinases and AKT in response to PDGF-BB stimulation between pig arterial smooth muscle cells (ApSMCs) and venous smooth muscle cells (VpSMCs). (A,E) Confluent cells were serum-starved overnight and stimulated with PDGF-BB at the indicated time points. Cell lysates were analyzed for the expression of the indicated proteins via immunoblots. (B–D,F) Individual band densitometry data were obtained using Image J (NIH version 1.53K). The ratio values were obtained by dividing the band densitometry values of pERK1/2 (B), pJNK (C), pP38 (D) and pAKT (F) by the loading protein values (n = 4 for pERK1/2 and pJNK; n = 3 for pP38; n = 5 for pAKT). ****: p < 0.0001; ***: p < 0.001; **: p < 0.01; *: p < 0.05; ns: non-significant differences were assessed using an ANOVA test.

    Article Snippet: The anti-ILK (3862), anti-TIMP-2 (5738), anti-pERK1/2(9101), anti-pJNK (9251), anti-pP38 (9211) and anti-pAKT (Serine 473) (9271) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Techniques: Activation Assay, Expressing, Western Blot

    Antibodies used in this study

    Journal: The Journal of Biological Chemistry

    Article Title: Tyrosine phosphorylation of the scaffold protein IQGAP1 in the MET pathway alters function

    doi: 10.1074/jbc.RA120.015891

    Figure Lengend Snippet: Antibodies used in this study

    Article Snippet: pAKT Ser-473 , Cell Signaling Technology, 4060S , 1:1000.

    Techniques: Immunoprecipitation